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| MS-Xelerator快速質(zhì)譜數(shù)據(jù)處理系統(tǒng) |
| 由于樣品制備和數(shù)據(jù)采集的自動(dòng)化，使得各種分析產(chǎn)生的LC-MS數(shù)據(jù)量急劇增加，從一系列數(shù)據(jù)中找出所有特征組分將是一項(xiàng)非常耗時(shí)的工作。 |
| MS-Xelerator能處理各種LC-MS、GC-MS、CE-MS數(shù)據(jù)，從中發(fā)現(xiàn)有意義的峰，具有無法比擬的處理速度和靈敏度......[全文] |
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為什么選擇MS-Xelerator快速質(zhì)譜數(shù)據(jù)系統(tǒng)？ 高速分析、性價(jià)比最高、用戶體驗(yàn)友好 簡(jiǎn)單易學(xué)，對(duì)操作者無特殊要求 市場(chǎng)上速度最快的軟件 非?？焖佟⑷娴馁|(zhì)譜數(shù)據(jù)分析軟件 同時(shí)適合LC-MS、GC-MS、CE-MS數(shù)據(jù) 圖形化顯示結(jié)果 快速強(qiáng)大的峰挑選算法和獨(dú)特的過濾算法 代謝組學(xué)、定量蛋白質(zhì)組學(xué)、雜質(zhì)分析、差異分析 生物標(biāo)記物發(fā)現(xiàn)的理想工具 |
| 蛋白質(zhì)分離、純度檢測(cè)和定量分析 |
| 雜質(zhì)輪廓（譜）分析 |
| 降解產(chǎn)物輪廓（譜）分析 |
| 代謝產(chǎn)物輪廓（譜）分析 |
| 差異分析 |
| 代謝組學(xué) |
| 定量蛋白質(zhì)組學(xué) |
| 生物標(biāo)記物發(fā)現(xiàn) |
‘We successfully used the Reference Peak Warping (RPW) function of the MsXelerator software to align profiles of rat-urinary metabolites obtained by capillary electrophoresis–mass spectrometry. The excellent alignment allowed reliable principal component analysis revealing significant differences between antibiotic-treated and non-treated rats.’ Miranda de Kok, PhD, Biomedical Analysis, Dept. of Pharmaceutical Sciences, Faculty of Science University Utrecht, the Netherlands
“我們使用MsXelerator軟件的RPW功能成功地校準(zhǔn)了大鼠尿液代謝物的毛細(xì)管電泳-質(zhì)譜數(shù)據(jù)。MsXelerator出色的校準(zhǔn)功能保證了后續(xù)的主成分分析得以成功揭示抗生素治療組和非治療組大鼠尿液代謝物顯著差異?！?Miranda de Kok，博士，生物醫(yī)學(xué)分析系，藥學(xué)院，烏德勒支大學(xué)，荷蘭‘We really enjoy working with MsMetrix to implement MsXelerator software according to our needs.? MsXelerator software, especially the part of the IPeaks module is a very powerful tool to process our routine reactive metabolite trapping data.’ Chenghong Zhang, Genentech (A Member of Roche), San Francisco, USA
“我們很樂意根據(jù)我們的需求使用MsMetrix公司的MsXelerator軟件實(shí)施我們的工作。MsXelerator軟件，尤其是IPeaks模塊，是一個(gè)處理常規(guī)活性代謝物捕集數(shù)據(jù)的強(qiáng)大工具?！?張成洪，Genentech公司（羅氏公司的一員），舊金山，美國‘MsXelerator enabled us to select peptides from very complex mixtures by recognition of their specifically induced isotope pattern. Manual inspection of thousands of spectra has been replaced by an hour of analysis time using MsXelerator.’ Dr. P.A. van Veelen, LUMC, Head Mass Spectrometry Group, Leiden, the Netherlands
“通過MsXelerator，我們可以從非常復(fù)雜的混合肽中識(shí)別出具有特異誘導(dǎo)同位素模式的肽。應(yīng)用MsXelerator分析 1小時(shí)就可以替代我們?nèi)斯こ汕先f次的光譜檢測(cè)工作?！?P.A.van Veelen，博士，萊頓大學(xué)醫(yī)學(xué)中心，質(zhì)譜主席，萊頓，荷蘭‘At Evolva SA, we have been using MsXelerator with great success for more than 4 years. Finding unknown molecules in complex sample matrix requires challenging and extensive LC/MS data mining, without mentioning the time needed to review high throughput screening data. MsXelerator software and particularly the module MPeaks does a great job in deconvoluting and comparing MS chromatograms in order to find the small differences which are relevant for our applications. Moreover, the clever interactivity between MPeaks and MS-compare help us accelerating our analysis thanks to the multiple filters and statistical tools, all with great confidence. Beyond all doubt, MsXelerator is a powerful all-in-one software platform to process LC/MS data, removing the hassle of buying several expensive add-ons from the instrument vendors. I also particularly enjoy the collaboration, expertise and quick responsiveness of MsMetrix, regarding customization to our special requests! ’ Christophe Folly, Research Scientist, Evolva SA, Switzerland
“在Evolva SA公司，4年前我們已開始使用MsXelerator，至今已經(jīng)獲得了巨大的成功。在復(fù)雜的樣品中尋找未知的分子，需要面臨的挑戰(zhàn)是深度的LC / MS數(shù)據(jù)挖掘以及高通量的數(shù)據(jù)篩選所需要的時(shí)間。MsXelerator 軟件特定的MPeaks模塊在解卷積和比對(duì)質(zhì)譜數(shù)據(jù)以及找尋和我們應(yīng)用相關(guān)的最小差異方面做得很好。此外，MPeaks 和MS-compare靈活的交互性幫助我們加速分析速度，其多重過濾和統(tǒng)計(jì)分析工具給了我們巨大的信心。毫無疑問，對(duì)于處理LC / MS數(shù)據(jù)，MsXelerator是一個(gè)集強(qiáng)大功能于一身的軟件平臺(tái)。除了幾個(gè)昂貴的組件需要從儀器供應(yīng)商購買比較麻煩之外，我還特別欣賞MsMetrix公司的協(xié)作力、專業(yè)性以及對(duì)于我們特殊需求的快速響應(yīng)力。” Christophe Folly，研究員，Evolva SA公司，瑞士‘MsXelerator is a real time-saver when processing our discovery and (pre)clinical Met-ID experiments at MSD (Schering-Plough, Organon).’ One workflow in a multi vendor ms lab. Searches for artificial isotope patterns at low level that worked (labeled compounds). Customization. Peter Jacobs, Head ADME Analysis, Schering-Plough
“在處理藥物發(fā)現(xiàn)和臨床Met-ID研究的質(zhì)譜實(shí)驗(yàn)數(shù)據(jù)時(shí)，MsXelerator可以真正為我們節(jié)省時(shí)間（Schering-Plough公司，Organon）。” ·在多個(gè)供應(yīng)商的MS實(shí)驗(yàn)室實(shí)現(xiàn)一個(gè)工作流程 ·搜索工作在較低水平的人造同位素模式（標(biāo)記化合物） ·定制 Peter Jacobs，藥物動(dòng)力學(xué)分析主席，Schering-Plough‘MsXelerator is an essential tool nowadays in processing our proteomics datasets. It provides sensitive algorithms to search for mass spectral doublets, either targeting absolute mass differences (in the context of e.g. SILAC-based proteomics experiments or chemical modifications of amino acids) or relative mass differences. This latter feature is unique in its kind, as it allocates14N/15N-isotopically labeled peptides characterized by a relative mass difference of 1.2% of the m/z-value of the peptide and user-specified peak intensity ratios. For our Immuno Proteomics research we often apply 14N/15N-metabolic labeling protocols to target MHC-presented epitopes. MsXelerator is exceptional in allocating these typical mass spectral doublets, based on relative mass differences, charge states and intensity ratios. All data processing is performed on the raw MS data, without any prior data conversion. MsXelerator also supports high resolution MS data (e.g. Orbitrap datasets) that significantly reduces the false positive hits and speeds up the automated processing of our complex and large data sets. The flexibility of the user interface, allows us to set and define many search parameters, depending on the design of the experiment. MsXelerator also features a good built-in browser to view the raw data (chromatograms, extracted ion chromatograms, mass spectra) without switching back and forth to the MS-vendor originating data system. It is without any doubt that this application has boosted our Immuno Proteomics research. Moreover, the good hands-on training and the direct contact with the software developers of MsXelerator allows us to have user-defined modules integrated in the basic version of the application. ’ Hugo D. Meiring, PhD, Scientist Biomolecular Mass SpectrometryUnit Vaccinology, Centre for Infectious Disease Control Netherlands, National Institute for Public Health and the Environment.
“MsXelerator是我們目前處理蛋白質(zhì)組學(xué)數(shù)據(jù)集的一個(gè)重要的工具。它提供了靈敏的算法來搜索質(zhì)譜中的雙峰，同時(shí)也適用于絕對(duì)質(zhì)量差異（例如：基于SILAC的定量蛋白質(zhì)組學(xué)實(shí)驗(yàn)或化學(xué)修飾的氨基酸）和相對(duì)質(zhì)量差異。 后者的特點(diǎn)是獨(dú)一無二的，其特點(diǎn)在于14N/15N同位素標(biāo)記肽的分配，以m / z值的相對(duì)質(zhì)量差為1.2％的肽和和用戶指定的峰強(qiáng)度比為特征。我們?cè)诿庖叩鞍踪|(zhì)組學(xué)的研究中，通常采用14N/15N代謝標(biāo)記目標(biāo)MHC的抗原決定簇。根據(jù)相對(duì)質(zhì)量差、電荷狀態(tài)和強(qiáng)度比，MsXelerator在分配典型的質(zhì)譜雙峰時(shí)是很完美的。可對(duì)所有的原始MS數(shù)據(jù)進(jìn)行處理，無需任何預(yù)處理。 MsXelerator還支持高分辨率質(zhì)譜數(shù)據(jù)（例如：Orbitrap的數(shù)據(jù)集），顯著地降低了假陽性出現(xiàn)，加快了自動(dòng)處理復(fù)雜和龐大數(shù)據(jù)集的速度。用戶界面靈活，允許我們根據(jù)實(shí)驗(yàn)的設(shè)計(jì)，設(shè)置并定義檢索參數(shù)。 MsXelerator還擁有一個(gè)內(nèi)置的瀏覽器用于直接查看原始數(shù)據(jù)（色譜圖、離子色譜圖、質(zhì)譜），無需切換至質(zhì)譜供應(yīng)商提供的原始數(shù)據(jù)系統(tǒng)查看數(shù)據(jù)。 毫無疑問，這個(gè)應(yīng)用程序提升了我們?cè)诿庖叩鞍踪|(zhì)組學(xué)上的研究。此外，良好的培訓(xùn)以及與MsXelerator軟件開發(fā)人員的直接聯(lián)系讓我們可以在基礎(chǔ)版本的基礎(chǔ)上擁有用戶自定義的集成模塊。” Hugo D. Meiring，博士，分子生物學(xué)、單位疫苗學(xué)質(zhì)譜方向的科學(xué)家，來自荷蘭傳染病控制中心國家公共衛(wèi)生和環(huán)境研究所。